444213

MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast

• Sinônimo(s): Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase
• Número da licença da enzima: 3.4.24.24 (BRENDA, IUBMB)
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Ensaio: ≥90% (SDS-PAGE)
• Nível de qualidade: 100
• forma: liquid
• atividade específica: ≥600 mU/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• solubilidade: aqueous buffer: soluble
• atividade externa: other MMP activity, none detected
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use.

Note: 1 mU = 1 milliunit.

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

forma física

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Nota de preparo

Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C).

Outras notas

Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.
Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.
Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.

Informações legais

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable