391338

HEPES

≥99% dry basis (titration), crystalline powder, amniosulfonic acid buffer

• Sinônimo(s): Ácido 4-(2-hidroxietil)piperazina-1-etanossulfônico, Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
• Fórmula empírica (Notação de Hill): C₈H₁₈N₂O₄S
• Número CAS: 7365-45-9
• Peso molecular: 238.30
• Beilstein: 883043
• Número MDL: MFCD00006158
• Código UNSPSC: 12352106
• NACRES: NA.25

Propriedades

• product name: HEPES, ULTROL^® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer
• Nível de qualidade: 100
• Ensaio: ≥99% dry basis (titration)
• forma: crystalline powder
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: white
• faixa de pH útil: 6.8-8.2
• pKa (25 °C): 7.5
• solubilidade: water: 1 M
• traços de ânion: chloride (Cl^-): ≤0.01%; sulfate (SO₄^2-): ≤0.05%
• traços de cátion: Na: ≤0.1%; heavy metals: ≤1 ppm
• Condições de expedição: ambient
• temperatura de armazenamento: 15-25°C
• cadeia de caracteres SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• chave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descrição

Descrição geral

A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pK_a of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.

A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pK_a of 7.48 at 25°C.

Absorbance (1.0 M, H₂O, 260 nm):≤0.05.

Absorbance (1.0 M, H₂O, 280 nm): ≤0.04

Advertência

Toxicity: Irritant (B)

Reconstituição

Following reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable