341583

FGF-2, Basic, Bovine

• Sinônimo(s): FGF-2, Basic, Bovine, nbbFGF, Fibroblast Growth Factor, Basic, Bovine
• Número CAS: 106096-93-9
• Número MDL: MFCD00081382
• Código UNSPSC: 12352202

Propriedades

• Ensaio: ≥97% (SDS-PAGE)
• Nível de qualidade: 100
• Formulário: lyophilized
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Impurezas: <0.1 ng/μg Endotoxin (ng/μg FGF)
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. A multifunctional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Known to activate phopholipase D in endothelial cells. Higher concentrations are found in the brain and pituitary gland. Purified from bovine brain by sequential chromatography.

Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Activates phospholipase D in endothelial cells.

Ações bioquímicas/fisiológicas

ED₅₀ = 50-150 pg/ml, in the absence of heparin, for stimulation of ³H-thymidine incorporation by NR6R-3T3 fibroblasts

Advertência

Toxicity: Standard Handling (A)

forma física

Lyophilized from sterile-filtered 20 mM Tris-HCl, 1M NaCl, 50 µg BSA/µg FGF, pH 7.0.

Reconstituição

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.

Outras notas

Ohmiya, M., et al. 2001. J. Neurosci. Res.65, 228.
Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
Thomas, K. 1987. Methods Enzymol.147, 120.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3