268298
DAPI, Dihydrochloride
Cell-permeable DNA-binding dye.
Sinônimo(s):
DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl
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About This Item
Fórmula empírica (Notação de Hill):
C16H15N5 · 2HCl
Número CAS:
Peso molecular:
350.25
Número MDL:
Código UNSPSC:
12352200
NACRES:
NA.54
Produtos recomendados
Nível de qualidade
Ensaio
≥90% (HPLC)
forma
solid
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
desiccated (hygroscopic)
protect from light
cor
yellow
solubilidade
water: 5 mg/mL
ethanol: soluble
methanol: soluble
fluorescência
λex ~359 nm
λem ~461 nm
Condições de expedição
ambient
temperatura de armazenamento
−20°C
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
chave InChI
FPNZBYLXNYPRLR-UHFFFAOYSA-N
Descrição geral
Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Ações bioquímicas/fisiológicas
Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Binds preferentially to DNA rich in adenine and thymine
Product does not compete with ATP.
Reversible: yes
Advertência
Toxicity: Standard Handling (A)
Reconstituição
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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