208772

Calpain-1 Substrate II, Fluorogenic

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides.

• Sinônimo(s): Calpain-1 Substrate II, Fluorogenic, H-E(EDANS)PLF~AERK(DABCYL)-OH
• Fórmula empírica (Notação de Hill): C₇₂H₉₇N₁₇O₁₆S
• Peso molecular: 1488.71
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥97% (HPLC)
• Formulário: lyophilized solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic); protect from light
• cor: red
• solubilidade: DMSO: 5 mg/mL
• fluorescência: λ_ex ~505 nm; λ_em ~335 nm
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively.

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Excitation: ~335 nm; emission: ~505 nm.

Ações bioquímicas/fisiológicas

Cell permeable: no

Primary Target
Calpain-1 Substrate

Product does not compete with ATP.

Reversible: no

Embalagem

Packaged under inert gas

Advertência

Toxicity: Irritant (B)

Sequência

H₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H

forma física

Supplied as a trifluoroacetate salt.

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Outras notas

Cuerrier, D., et al. 2007. J. Biol. Chem.282, 9600.
Cuerrier, D., et al. 2005. J. Biol. Chem.280, 40632.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable