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Key Documents

1.52252

Supelco

Chromolith® WP 300 Epoxy (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Sinônimo(s):

Chromolith® WP 300 Epoxy 25-4.6 HPLC column

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

product name

Chromolith® WP 300 Epoxy, L × I.D. 25 mm x 4.6 mm HPLC column

Materiais

PEEK column

Nível de qualidade

linha de produto

Chromolith®
Chromolith®

Características

endcapped: no
endcapped: no

embalagem

1 ea of

Extensão da rotulagem

5-6% Carbon loading

Parâmetros

2 - 60 °C temp. range
2-60 °C temp. range
200 bar pressure
200 bar pressure

técnica(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable

coluna C × D.I.

2.5 cm × 4.6 mm

área da superfície

120 m2/g

matriz

monolithic silica
monolithic silica

Grupo ativo da matriz

Epoxy bonding phase
Epoxy bonding phase

tamanho de poro

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

operating pH range

1.5-7.5
1.5-7.5

temperatura de armazenamento

no temp limit

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Descrição geral

Chromolith® WP 300 Epoxy columns are specially designed for the user-specific immobilization of ligands and their later application in HPLC. The unique bimodal pore structure of silica monoliths allows efficient coupling independent of molecule size. The wider mesopores also enable the use of proteins and antibodies as both ligand immobilized on the column, and later analyte separated by an immobilized column.
Potential applications: attach trypsin to obtain HPLC column-protein digestion reactor; attach protein and measure other protein interaction with the attached one; attach any chiral selector to obtain a chiral column; attach any affinity ligand to obtain custom made affinity column, etc.

Chromolith® WP 300 Epoxy 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Easy to perform immobilization of ligand opens up limitless customization options
—Monolithic format allows for high flow rates at low backpressure, enabling high throughput

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Epoxy is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Create Your Own Column Chemistry - Chromolith® WP 300 Epoxy 2 mm I.D. HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Informações legais

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análise (COA)

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Artigos

Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.

Conteúdo relacionado

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Chromolith® WP 300 Epoxy: Learn more about preparing the column, immobilization via epoxide functions and via Schiff base mechanism & use as HPLC column.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

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