14-276-M
Akt1/PKBα Protein, active, 15 µg
Active, recombinant full-length human Akt1, containing N-terminal His6 tag, for use in IP-Kinase Assays & Kinase Assays.
Sinônimo(s):
Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha
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About This Item
Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.26
Produtos recomendados
fonte biológica
human
Nível de qualidade
recombinante
expressed in baculovirus infected Sf21 cells
forma
liquid
prazo de validade
1 yr
peso molecular
Mw 59.9 kDa
fabricante/nome comercial
Upstate®
técnica(s)
activity assay: suitable (IP-kinase)
activity assay: suitable (kinase)
solubilidade
soluble
nº de adesão NCBI
nº de adesão UniProt
temperatura de armazenamento
−70°C
Informações sobre genes
human ... AKT1(207)
Descrição geral
Research area: CELL SIGNALING
Product Source: Expressed by baculovirus in Sf21 insect cells
Recombinant full-length human Akt1, containing N-terminal His6-tag.
Product Source: Expressed by baculovirus in Sf21 insect cells
Recombinant full-length human Akt1, containing N-terminal His6-tag.
Ações bioquímicas/fisiológicas
Protein Target: Akt1/PKBα
Target Sub-Family: AGC
Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.
Target Sub-Family: AGC
Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.
Embalagem
Also available in 250μg size, please inquire for pricing and availability.
Qualidade
Routinely evaluated by phosphorylation of Crosstide. Please refer to the Certificate of Analysis for more information.
forma física
Ni2+/NTA and glutathione agarose
Supplied in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.
Armazenamento e estabilidade
1 year at -70°C
Outras notas
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe de risco de água (WGK)
WGK 2
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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A common phosphate binding site explains the unique substrate specificity of GSK3 and its inactivation by phosphorylation
Frame, S., et al
Molecular Cell, 7, 1321-1327 (2001)
Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.
Miinea, Cristinel P and Lienhard, Gustav E
Biotechniques, 34, 828-831 (2003)
B Bauer et al.
The Journal of biological chemistry, 276(34), 31627-31634 (2001-06-19)
Protein kinase C theta (PKC theta) is known to induce NF-kappa B, an essential transcriptional element in T cell receptor/CD28-mediated interleukin-2 production but also T cell survival. Here we provide evidence that PKC theta is physically and functionally coupled to
Madhavi J Rane et al.
The Journal of biological chemistry, 278(30), 27828-27835 (2003-05-13)
Activation of the serine-threonine kinase Akt by cytokines, chemokines, and bacterial products delays constitutive neutrophil apoptosis, resulting in a prolonged inflammatory response. We showed previously that Akt exists in a signaling complex with p38 MAPK, MAPK-activated protein kinase-2 (MAPKAPK-2), and
P Cohen et al.
FEBS letters, 410(1), 3-10 (1997-06-23)
The initial steps in insulin signal transduction occur at the plasma membrane and lead to the activation of phosphatidylinositide (PtdIns) 3-kinase and the formation of PtdIns(3,4,5,)P3 in the inner leaflet of the plasma membrane which is then converted to PtdIns(3,4)P2
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