1.25181

Fractogel^® EMD SO₃⁻ (S) Resin

MiniChrom Column Fractogel^® SO3(S), volume 1 mL, prepacked column

• Sinônimo(s): MiniChrom Fractogel^® SO3(S) 1 ml
• Código UNSPSC: 41115711

Propriedades

• ligand: (Sulfoisobutyl)
• Nível de qualidade: 100
• Materiais: polypropylene housing
• linha de produto: Fractogel^®
• forma: prepacked column
• Parâmetros: ≤8 bar pressure; ~80 cm/hr max. flow rate
• column I.D. × L: 8 mm × 20 mm
• volume: 1 mL
• Grupo ativo da matriz: Methacrylate
• tamanho médio de partícula: 20-40 μm
• capacidade: ~150 mL, resin binding capacity
• aplicação(ões): recombinant protein; vaccine development
• temperatura de armazenamento: no temp limit

Descrição

Descrição geral

8 x 20mm

Características e benefícios

MiniChrom Fractogel^® SO3(S) 1 ml enables:

• Efficient separation between monomer, dimer, and high molecular weight aggregates
• High binding capacity
• High resolution
• Efficient polishing step
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (S) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Embalagem

Plastic bag(s)

Informações legais

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany