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Key Documents

1.11278

Millipore

M FC agar

GranuCult® plus, suitable for microbiology, for coliforms

Sinônimo(s):

Membrane Fecal Coliform Agar, m-Fecal Coliform Agar

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About This Item

Código UNSPSC:
41106213
NACRES:
NA.74

forma

granular

Nível de qualidade

embalagem

pkg of 500 g

fabricante/nome comercial

GranuCult® plus

condição de armazenamento

dry media (Store tightly closed)

técnica(s)

microbiological culture: suitable

cor

blue

pH

7.2-7.6 (25 °C, 52 g/L in H2O)

solubilidade

52 g/L

aplicação(ões)

bioburden testing
food and beverages

microbiology

temperatura de armazenamento

15-25°C

adequação

coliforms

Descrição geral

m-FC Agar is a selective membrane filtration medium suitable for the detection of fecal coliforms. The fecal coliforms derived from the intestinal tract of warm-blooded animals are differentiated from the other environmental coliforms due to their ability to grow at higher temperatures. The medium is supplemented with rosolic acid and incubated at 44.5 °C ± 0.2 °C for 24 h. Peptone and yeast extract, which serve as nutritious sources, and bile salts are added to inhibit accompanying Gram-positive flora. Lactose can be fermented by fecal coliforms at elevated temperatures to form blue colonies on the prepared medium (agar base with rosolic acid), whereas other organisms form grey colonies.

Aplicação

m-FC Agar is used for the cultivation and enumeration of fecal coliforms by the membrane filtration technique.

Características e benefícios

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Nota de análise

Appearance (clearness): clear
Appearance (colour): blue to violet
pH-value (25 °C): 7.2 - 7.6
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good; colony colour blue to dark blue
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good; colony colour pink to red
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): no growth
Growth (Enterobacter aerogenes ATCC 13048): good; colony colour grey to grey-blue
Incubation: 24 hrs.; 44.5 °C; aerobic.

Nota de rodapé

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3


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