1.04134

Eosina Y (amarelada) (C.I. 45380)

• Fórmula empírica (Notação de Hill): C₂₀H₆Br₄Na₂O₅
• Número CAS: 17372-87-1
• Peso molecular: 691.85
• Número MDL: MFCD00005040
• Código UNSPSC: 41116130
• NACRES: NA.21

Propriedades

• Formulário: solid
• Nível de qualidade: 200
• potência: >2000 mg/kg LD₅₀, oral (Rat)
• composição: Dye, ≥85% spectrophotometric assay
• pH: 9.2 (20 °C, 10 g/L in H₂O)
• pf: 295-296 °C
• solubilidade: 300 g/L
• densidade volumétrica: 710 kg/m³
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: 2-30°C
• cadeia de caracteres SMILES: [Na+].[Na+].Brc1c2[o][c]3[c](c(c2cc([c]1=O)Br)c4c(cccc4)C(=O)[O-])=CC(=C(C=3Br)[O-])Br
• InChI: 1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• chave InChI: SEACYXSIPDVVMV-UHFFFAOYSA-L

Descrição

Descrição geral

Eosin Y (C.I. 45380) is a CERTISTAIN^® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin Y staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange. As the Eosin Y dye belongs to the portfolio of Certistain^® dyes, it is chemically analyzed according to strict specifications before release and a high staining quality, with reliable staining results is guaranteed.

Aplicação

for microscopy Certistain^®

Nota de análise

Dye content (gravimetrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 515 - 518 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; water): 1200 - 1400
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Informações legais

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317 - H319
• Declarações de precaução: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 376.9 °F - Pensky-Martens closed cup
• Ponto de fulgor (°C): 191.6 °C - Pensky-Martens closed cup