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Documentos Principais

1.01603

Sigma-Aldrich

Gram-Color modified

(phenol-free) staining kit for Gram staining method on bacteriological smears

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About This Item

Código UNSPSC:
41116130
NACRES:
NA.74

Nível de qualidade

não contém

phenol

IVD

for in vitro diagnostic use

técnica(s)

Gram staining: suitable

aplicação(ões)

clinical testing
diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

15-25°C

Descrição geral

The Gram-Color modified (phenol-free) - staining kit for Gram staining method on bacteriological smears, is used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin from smears of body fluids. Gram-Color modified is a staining kit which is used for a modified Gram staining. The Gram-Color modified solutions are modified and designed in such a way that staining can be carried out on the staining rack. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet an aniline dye. After the treatment with iodine solution (Lugol′s solution), a dye-iodine complex will form. During the decolorizing step, this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with fuchsin solution and will then appear red.
The kit is sufficient for up to 65-70 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Nota de análise

Suitability for microscopy: passes test
gram-positve microorganisms: dark blue
gram-negative microorganisms: red

Palavra indicadora

Danger

Classificações de perigo

Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - STOT SE 3

Órgãos-alvo

Central nervous system

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

14.0 °F

Ponto de fulgor (°C)

-10 °C


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