06-445

Anti-Kip1 (p27) Antibody

Upstate^®, from rabbit

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: rabbit, human, rat
• embalagem: antibody small pack of 25 μg
• fabricante/nome comercial: Upstate^®
• técnica(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NM_004064
• nº de adesão UniProt: P46527
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CDKN1B(1027)

Descrição

Descrição geral

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.

Especificidade

Recognizes Kip1.

Imunogênio

An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits

Aplicação

Anti-Kip1 (p27) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Kip1 (p27) & has been validated in IHC, IP & WB.

Qualidade

routinely evaluated by immunoblot on HeLa nuclear extract or in rabbit brain nuclear extract

Descrição-alvo

27 kDa

Ligação

Replaces: 04-240

forma física

Format: Purified

Nota de análise

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany