05-432
Anti-NR1 Antibody, CT
Upstate®, from mouse
Sinônimo(s):
Grin1, NMDA R1 receptor C1 cassette, glutamate receptor, ionotropic, N-methyl D-aspartate 1
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
monoclonal
reatividade de espécies
rat
reatividade da espécie (prevista por homologia)
human, mouse
fabricante/nome comercial
Upstate®
técnica(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... GRIN1(2902)
rat ... Grin1(24408)
Descrição geral
NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
Especificidade
This antibody is specific for NR1, Mr 130 kDa. The epitope maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms. An additional band may be detected at higher concentrations, Mr 200kDa.
Imunogênio
Epitope: The epitope maps to a region (a.a. 834-864) common to all NR1 isoforms
His-tag fusion protein corresponding to residues 834-938 of rat NR1A. The immunizing sequence is identical in rat and human.
Aplicação
Anti-NR1 Antibody, CT detects level of NR1 & has been published & validated for use in IC, IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.
Immunocytochemistry:
An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.
Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.
4 μg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.
Immunocytochemistry:
An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.
Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.
Qualidade
Routinely evaluated by Western Blot on rat brain microsomal protein preparation.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected NR1 from 20 μg of rat brain microsomal protein preparation (Catalog # 12-144).
Western Blot Analysis:
0.5-2 μg/mL of this lot detected NR1 from 20 μg of rat brain microsomal protein preparation (Catalog # 12-144).
Descrição-alvo
130 kDa observed
forma física
Format: Purified
Purified mouse monoclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2° to 8ºC from date of receipt.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Glutamate receptor modulation by protein phosphorylation.
Raymond, L A, et al.
Journal of Physiology, Paris, 88, 181-192 (1994)
Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors.
She, K; Ferreira, JS; Carvalho, AL; Craig, AM
The Journal of Biological Chemistry null
C M Forrest et al.
Neuroscience, 310, 91-105 (2015-09-15)
Glutamate receptors sensitive to N-methyl-D-aspartate (NMDA) are involved in embryonic brain development but their activity may be modulated by the kynurenine pathway of tryptophan metabolism which includes an agonist (quinolinic acid) and an antagonist (kynurenic acid) at these receptors. Our
Jamie E Sheren et al.
Biochemical and biophysical research communications, 440(4), 473-478 (2013-08-27)
The RNF38 gene encodes a RING finger protein of unknown function. Here we demonstrate that RNF38 is a functional ubiquitin protein ligase (E3). We show that RNF38 isoform 1 is localized to the nucleus by a bipartite nuclear localization sequence
Natural reward experience alters AMPA and NMDA receptor distribution and function in the nucleus accumbens.
Pitchers, KK; Schmid, S; Di Sebastiano, AR; Wang, X; Laviolette, SR; Lehman, MN; Coolen, LM
Testing null
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