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Documentos Principais

05-368

Sigma-Aldrich

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody

clone MPM-2, Upstate®, from mouse

Sinônimo(s):

Phospho-Ser/Thr Antibody

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

clone

MPM-2, monoclonal

reatividade de espécies

vertebrates

fabricante/nome comercial

Upstate®

técnica(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1

Condições de expedição

wet ice

modificação pós-traducional do alvo

phosphorylation (pSer/pThr)

Descrição geral

This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.

Especificidade

Recognizes phosphorylated serines or threonines when followed by a proline.

Imunogênio

Mitotic human HeLa cell cytosolic lysate

Aplicação

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in ELISA, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualidade

routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells

Descrição-alvo

varies depending on the protein you are detecting

forma física

Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Armazenamento e estabilidade

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análise

Control
Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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S A Innocente et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(5), 2147-2152 (1999-03-03)
The p53 tumor suppressor controls multiple cell cycle checkpoints regulating the mammalian response to DNA damage. To identify the mechanism by which p53 regulates G2, we have derived a human ovarian cell that undergoes p53-dependent G2 arrest at 32 degrees
J Cobb et al.
Developmental biology, 205(1), 49-64 (1999-01-12)
Little is known about the timing of meiotic prophase events during spermatogenesis in the mouse or how these events are related to cell-cycle progression. This work was designed to test hypotheses about the timing and biochemical correlates of developmental acquisition
Cyclin E controls Drosophila female germline stem cell maintenance independently of its role in proliferation by modulating responsiveness to niche signals.
Ables, ET; Drummond-Barbosa, D
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Mitotic mechanisms in Alzheimer's disease?
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