05-1000

Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)

clone 4A4, Upstate^®, from mouse

• Sinônimo(s): Clone 4A4 Anti-Phosphoserine, Phosphoserine Detection Antibody
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 4A4, monoclonal
• reatividade de espécies: vertebrates
• fabricante/nome comercial: Upstate^®
• técnica(s): ELISA: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: phosphorylation (pSer)

Descrição

Descrição geral

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%).

Especificidade

This antibody recognizes serine-phosphorylated proteins from all species.

Imunogênio

Phosphoserine coupled to KLH.

Aplicação

Detect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB.

Immunofluorescence

Flow Cytometry

Immunohistochemistry (Paraffin)

ELISA

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Qualidade

Routinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.

Western Blot Analysis:
0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.

Descrição-alvo

Dependent upon the molecular weight of the serine phosphorylated protein being detected.

forma física

Format: Purified

Protein G-Sepharose Chromatography

Purified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol.

Armazenamento e estabilidade

Stable for 1 year at -20ºC from date of receipt.
For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable