05-172-AF488

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^™ 488 Conjugate

clone Sm1.2, from mouse, ALEXA FLUOR^™ 488

• Sinônimo(s): Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• conjugado: ALEXA FLUOR^™ 488
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: Sm1.2, monoclonal
• reatividade de espécies: chicken, rat, mouse, human, fish
• técnica(s): immunocytochemistry: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_000609
• nº de adesão UniProt: P05019
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... IGF1(3479)

Descrição

Descrição geral

Insulin-like growth factor I (UniProt P05019; also known as IGF-I, Somatomedin-C) is encoded by the IGF1 (also known as IGFI, MGF) gene (Gene ID 3479) in human. Human insulin-like growth factor-I (IGF-I) is a single chain 70 amino acid polypeptide that shares structural homology with insulin and human IGF-II. IGF-I plays a critical role in growth, differentiation and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-I is a regulator of metabolism and is also implicated in the pathophysiology of cancer, diabetes and obesity. Upon binding the tetrameric (alpha:beta)2 IGF-I receptor, the receptor alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.

Especificidade

IGF-I (Russel, W.E., (1984). Proc. Natl. Acad. Sci. U. S. A. 81(8):2389-2392). 40% cross reactivity to IGF-II (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522).

Imunogênio

Highly purified human plasma insulin-like growth factor I (IGF-I; Somatomedin C; Sm-C) conjugated to mouse albumin (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522). Action of somatomedins on cell growth: Effect of selective neutralisation of somatomedin C (IGF-I) with a monoclonal antibody. In: Raiti, S., Tolman, and R.A. (eds.), Human Growth Hormone. pp. 585–599. Plenum Publishing Corp., New York, NY).

Aplicação

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^™ 488 Conjugate is an antibody against IGF-I for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IGF-I in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-172) is shown to be suitable also for Western blotting, immunohistochemistry, immunoprecipitation, and neutralization applications.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Qualidade

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IGF-I in A431 cells.

Descrição-alvo

~17 kDa observed

forma física

Protein G Purified

Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações legais

ALEXA FLUOR is a trademark of Life Technologies

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable