05-1354

Anti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5

ascites fluid, clone 6HH3-2C5, from mouse

• Sinônimo(s): H3K9me2S10P, Histone H3 (di methyl K9, phospho S10), H3 histone, family 3B (H3.3B)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: ascites fluid
• tipo de produto de anticorpo: primary antibodies
• clone: 6HH3-2C5, monoclonal
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): Drosophila (based on 100% sequence homology)
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão GenBank: AK304607
• nº de adesão UniProt: B4E380
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: dimethylation (Lys9), phosphorylation (pSer10)
• Informações sobre genes: human ... H3F3B(3021)

Descrição

Descrição geral

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

Especificidade

Antibody recognizes dimethyl (Lys9) phospho(Ser10) on Histone H3.

Imunogênio

Epitope: Dimethyl (Lys9) & Phospho (Ser10)

Synthetic linear peptide corresponding to dimethyl (Lys9) and Phospho (Ser10) of Histone H3.

Aplicação

Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in A431 and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5 (mouse monoclonal antibody) validated in WB, ICC to detect Histone H3 (Dimethyl K9 also known as H3K9me2S10P, Histone H3 (di methyl K9 phospho S10).

Qualidade

Evaluated by western blot in untreated and Etoposide-treated HeLa acid extracts.

Western Blot Analysis:
A 1:1,000 dilution of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in 10 µg of untreated and Etoposide-treated HeLa acid extracts.

Descrição-alvo

~17 kDa

forma física

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Untreated and Etoposide-treated HeLa acid extracts.

Informações legais

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Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable