04-721

Anti-Dicer1 Antibody, clone 5D12.2

clone 5D12.2, Upstate^®, from mouse

• Sinônimo(s): DCR1, Dicer, HERNA, KIAA0928
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: 5D12.2, monoclonal
• application: WB
• reatividade de espécies: human
• técnica(s): western blot: suitable
• citations: 5

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 5D12.2, monoclonal
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μL
• fabricante/nome comercial: Upstate^®
• técnica(s): western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_085124.2
• nº de adesão UniProt: Q9UPY3
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... DICER1(23405)

Descrição

Descrição geral

RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).

Especificidade

Human Dicer1

Imunogênio

KLH conjugated synthetic peptide corresponding to amino acids 378-385 of human Dicer1

Aplicação

Anti-Dicer1 Antibody, clone 5D12.2 is a Mouse Monoclonal Antibody for detection of Dicer1 also known as DCR1, Dicer, HERNA, KIAA0928 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

RNA Binding Protein (RBP)

Western blot

Qualidade

Routinely evaluated by western blot on lysates from HeLa nuclear extracts.

Descrição-alvo

220 kDa

forma física

Format: Purified

Protein G Chromatography

Armazenamento e estabilidade

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Outras notas

feature_concentration_valuePlease refer to lot specific datasheet.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable