04-642
Anti-Ago2 Antibody, clone 9E8.2
ascites fluid, clone 9E8.2, Upstate®
Sinônimo(s):
Argonaute-2, Eukaryotic translation initiation factor 2C, AGO2, eIF-2C, Slicer protein, MGC3183, Piwi domain protein, PPD
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
9E8.2, monoclonal
reatividade de espécies
human
fabricante/nome comercial
Upstate®
técnica(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
Isotipo
IgG1κ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... AGO2(27161)
Descrição geral
Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).
Especificidade
human Ago2
Imunogênio
Epitope: near the N-terminus of Ago2
KLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG
Aplicação
Anti-Ago2 Antibody, clone 9E8.2 is a Mouse Monoclonal Antibody for detection of Ago2 also known as Argonaute-2, Eukaryotic translation initiation factor 2C & has been validated in WB and has been demonstrated to perform in ChIP and ChIP-seq.
ChIP and ChIP-seq: A representative lot of this antibody was used to perform ChIP and ChIP-seq ( Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Chromatin Biology
RNA Binding Protein (RBP)
RNA Metabolism & Binding Proteins
Chromatin Biology
RNA Binding Protein (RBP)
Qualidade
Routinely evaluated by immunoblot.
Descrição-alvo
~100 kda
forma física
Ascites with 0.05% sodium azide
ImmunoAffinity Purified
Armazenamento e estabilidade
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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