04-1578

Anti-Bruton′s tyrosine kinase (Btk) Antibody, clone 10D11

clone 10D11, from mouse

• Sinônimo(s): Agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase, Bruton agammaglobulinemia tyrosine kinase, Bruton tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, tyrosine-protein kinase BTK
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: 10D11, monoclonal
• application: IP; WB
• reatividade de espécies: rat, human
• técnica(s): immunoprecipitation (IP): suitable; western blot: suitable
• citations: 1

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 10D11, monoclonal
• reatividade de espécies: rat, human
• técnica(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_000052.1
• nº de adesão UniProt: Q06187
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... BTK(695)

Descrição

Descrição geral

Btk (Bruton’s tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. BTK plays a crucial role in B cell activation and development. Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner. Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA).

Especificidade

Recognizes human Btk, MW ~77 kDa.
A higher molecular weight protein may be detected, which is thought to be a phosphorylated species of Btk or a related protein.

Imunogênio

Epitope: Intracellular

GST-tagged recombinant protein corres-ponding to human Btk.

Aplicação

Detect Bruton′s tyrosine kinase (Btk) using this Anti-Bruton′s tyrosine kinase (Btk) Antibody, clone 10D11 validated for use in WB & IP.

Immunoprecipitation Analysis: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Btk in the presence of 0.5% SDS. Not recommended for IP/kinase assays.

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Qualidade

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.05- 1 µg/mL of this antibody detected Btk on 10 µg of Raji cell lysate.

Descrição-alvo

~77 kDa

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Raji cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable