04-1555

Anti-Mdmx Antibody, clone 8C6

clone 8C6, from mouse

• Sinônimo(s): Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse), Mdm4, transformed 3T3 cell double minute 4, p53 binding protein, Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mo
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 8C6, monoclonal
• reatividade de espécies: human
• técnica(s): ChIP: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2bκ
• nº de adesão NCBI: NP_002384.2
• nº de adesão UniProt: O15151
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MDM4(4194)

Descrição

Descrição geral

Mdmx is a Mdm2-related protein that like Mdm2 has been shown to bind to and inhibit p53, although not as well as Mdm2. This interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Mdmx has been shown to counteract Mdm2-dependent p53 degradation and to stabilize p53 in its inactive form. Although Mdmx has significant structural similarity to Mdm2 E3 ubiquitin ligase, most studies have not been able to ascribe E3 ligase function to Mdmx.

Especificidade

This antibody recognizes Mdmx.

Imunogênio

Epitope: Unknown

His-tagged recombinant protein corresponding to human Mdmx.

Aplicação

Anti-Mdmx Antibody, clone 8C6 is a Mouse Monoclonal Antibody for detection of Mdmx also known as MDM4-related protein 1, Mdm2-like p53-binding protein & has been validated in WB, IP, ICC, IHC, FC.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratoryfor this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)

Flow Cytometry Analysis: A previous lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualidade

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Mdmx on 10 µg of MCF-7 cell lysate.

Descrição-alvo

~ 80 kDa

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
MCF-7 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable