04-135

Anti-Brachyury Antibody, clone 3E4.2

clone 3E4.2, Upstate^®, from mouse

• Sinônimo(s): T brachyury (mouse) homolog, T brachyury-like, T protein, T, brachyury homolog (mouse), transcription factor T
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 3E4.2, monoclonal
• reatividade de espécies: human
• fabricante/nome comercial: Upstate^®
• técnica(s): western blot: suitable
• entrada: sample type mesenchymal stem cell(s)
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_003172
• nº de adesão UniProt: O15178
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TBXT(6862)

Descrição

Descrição geral

The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Especificidade

Reactivity with other species has not been tested.

This monoclonal antibody recognizes the brachyury protein.

Imunogênio

Epitope: C-terminus

Synthetic human brachyury peptide, a.a. 224-435.

Aplicação

Research Category
Stem Cell Research

Research Sub Category
Mesenchymal Stem Cells

This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.

Qualidade

Routinely evaluated by Western blot on NT2 cell lysates.

Western Blotting:
Recommended working dilutions are 1:1000.

Descrição-alvo

47 kDa

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Armazenamento e estabilidade

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable