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Key Documents

100600C

Avanti

E. coli Extract Polar

Avanti Polar Lipids

Sinônimo(s):

E. coli Polar Lipid Extract

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About This Item

Número CAS:
Código UNSPSC:
12352211
NACRES:
NA.25

forma

chloroform solution

embalagem

pkg of 2 × 20 mL (100600C-1g)
pkg of 1 × 4 mL (100600C-100mg)
pkg of 5 × 4 mL (100600C-500mg)

fabricante/nome comercial

Avanti Polar Lipids

concentração

25 mg/mL (100600C-100mg)
25 mg/mL (100600C-1g)
25 mg/mL (100600C-500mg)

tipo de lipídio

lipid extracts

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.

Aplicação

E. coli Extract Polar is suitable to prepare small unilamellar vesicles, to mimic the surface of the E. coli inner membrane.
E. coli extract polar has been for the preparation of liposomes.
E. coli Extract Polar has been used:
  • in unilamellar liposomes preparation for kinetic studies
  • in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies
  • in the reconstitution of molybdate transport protein (MolBC) for continuous wave-electron paramagnetic resonance studies

Ações bioquímicas/fisiológicas

E.coli Polar lipid extracts comprise phosphatidylethanolamine as major lipid component. The minor components include the phosphatidylglycerol (PG) and cardiolipin. The leaflets of cytoplasmic membrane and inner leaflet of outer membrane are the source of E. coli lipid extract. It is used for reconstituting membrane proteins and forms bilayer by vesicle fusion.

Embalagem

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100600C-1g)
5 mL Clear Glass Sealed Ampule (100600C-100mg)
5 mL Clear Glass Sealed Ampule (100600C-500mg)

Informações legais

Avanti Research is a trademark of Avanti Polar Lipids, LLC

geralmente comprado junto com este produto

Pictogramas

Skull and crossbonesHealth hazard

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Órgãos-alvo

Central nervous system

Código de classe de armazenamento

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Methods in Cell Biology, 128, 223-241 (2015)
Small substrate transport and mechanism of a molybdate ATP binding cassette transporter in a lipid environment
Rice AJ, et al.
The Journal of biological chemistry, 289(21), 15005-15013 (2014)
An inducible reconstitution system for the real-time kinetic analysis of protease activity and inhibition inside the membrane
Baker RP and Urban S
Methods in Enzymology, 584, 229-253 (2017)
The
Enzymology Primer for Recombinant DNA Technology (2017)
Effects of lipid environment on the conformational changes of an ABC importer
Rice AJ, et al.
Channels (Austin, Tex.), 8(4), 327-333 (2014)

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