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Documentos Principais

901756

Sigma-Aldrich

Low endotoxin gelatin from porcine skin

gel strength 240-360 (Bloom), <10 EU/g Endotoxin

Sinônimo(s):

beMatrix™ gelatin LS-H

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About This Item

Número CAS:
9000-70-8
Número MDL:
MFCD00564673
Código UNSPSC:
41116130
NACRES:
NA.23

Formulário

powder

Impurezas

<10 EU/g Endotoxin
<300 g Total viable aerobic count

perda

<15% loss on drying

cor

white to pale yellow

pH

5.0-6.5

viscosidade

4.0-6.5 mPa.s

traços de ânion

sulfite (SO32-): <1.5 mg/20g

traços de cátion

As: <1 ppm
Hg: <0.1 ppm
heavy metals: <20 ppm

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Aplicação

Gelatin has received significant attention in the biomedical field due to its inherent bioactivity. Low endotoxin gelatin from porcine skin, gel strength 300 bloom is a high bloom (high molecular weight) gelatin featuring less than 10 endotoxin units (EU) per gram. Endotoxins primarily consist of lipopolysaccharides from the outer membrane of gram-negative bacteria. Once in the bloodstream, endotoxins are capable of eliciting a strong immune response, potentially leading to fever, shock, a drop in blood pressure, and even potentially death. In addition to this immune response, several reports suggest endotoxins may also damage various types of cells. To avoid adverse effects, raw materials should contain the lowest level of endotoxins possible when used in biomedical or regenerative medicine research applications. This low endotoxin gelatin is derived from alkaline-treated porcine skin and has a variety of potential applications in the fields of regenerative medicine, medical devices, and tissue engineering research.

Informações legais

beMatrix is a trademark of Nitta Gelatin

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number

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Gelatin methacryloyl gel strength 300&#160;g Bloom, 80% degree of substitution

Sigma-Aldrich

900496

Gelatin methacryloyl

Gelatin from bovine skin gel strength ~225&#160;g Bloom, Type B

Sigma-Aldrich

G9382

Gelatin from bovine skin

Collagen Type IV from human cell culture Bornstein and Traub Type IV, 0.3&#160;mg/mL, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

C6745

Collagen Type IV from human cell culture

Gelatin from bovine skin Type B, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

G9391

Gelatin from bovine skin

Gelatin from bovine skin Type B

Sigma-Aldrich

G6650

Gelatin from bovine skin

K W Brunson et al.
Journal of supramolecular structure, 9(2), 231-242 (1978-01-01)
Chinese hamster ovary (CHO . K1 . PRO) cell growth was inhibited by addition of a gram-negative bacterial lipopolysaccharide (LPS) to the cell culture medium. Growth inhibition began after three or four days of incubation, was dose-dependent up to a
T Kirikae et al.
International journal of immunopharmacology, 19(5), 255-262 (1997-05-01)
Trace amounts of endotoxin (lipopolysaccharide: LPS) are assumed to contaminate commercially available fetal bovine serum (FBS) for tissue or cell culture during the manufacturing process. We examined how cultured cells were affected by the endotoxin and how much endotoxin was
J G Brock-Utne et al.
Anaesthesia and intensive care, 17(1), 49-55 (1989-02-01)
Endotoxins (lipopolysaccharides, LPS) are potent bacterial poisons always present within the intestines in considerable amounts. Several pathophysiological conditions such as hypovolaemia, hypoxia, intestinal ischaemia, burns and radiation lead to a breakdown in the barrier and depending upon the extent of
Shoko Yamamoto et al.
Journal of materials science. Materials in medicine, 28(3), 48-48 (2017-02-09)
Calcium phosphate cements (CPCs), consisting of a mixture of calcium phosphate powders and setting liquid, have been widely used in orthopedic applications. One of the drawbacks of CPCs is their poor resorbability in the living body, which hinders substitution with
E J Ziegler et al.
The New England journal of medicine, 307(20), 1225-1230 (1982-11-11)
In an effort to decrease deaths from gram-negative bacteremia and endotoxin shock, we treated bacteremic patients with human antiserum to endotoxin (lipopolysaccharide) core. Antiserum was prepared by vaccinating healthy men with heat-killed Escherichia coli J5; this mutant lacks lipopolysaccharide oligosaccharide
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