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U0880

Sigma-Aldrich

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Synonyme(s) :

Urate: oxygen oxoreductase

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About This Item

Numéro CAS:
9002-12-4
Numéro de classification (Commission des enzymes):
1.7.3.3 (BRENDA, IUBMB)
Numéro MDL:
MFCD00082126
Code UNSPSC :
12352204
ID de substance PubChem :
24900619
Nomenclature NACRES :
NA.54

Produit recombinant

expressed in E. coli

Forme

lyophilized powder

Activité spécifique

≥2 units/mg solid

Poids mol.

35 kDa by SDS-PAGE

Température de stockage

−20°C

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

Clé InChI

VPXDHZMLJOJKOX-UHFFFAOYSA-N

Description générale

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:
  • to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
  • to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
  • for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Actions biochimiques/physiologiques

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Définition de l'unité

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Forme physique

Powder; contains citrate and sucrose

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Journal of the American Society of Nephrology : JASN, 12(5), 1001-1009 (2001-04-24)
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Pediatrics, 131(1), e309-e312 (2012-12-05)
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Urate is the end product of purine metabolism and a major antioxidant circulating in humans. Recent data link higher levels of urate with a reduced risk of developing Parkinson's disease and with a slower rate of its progression. In this
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