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94310

Sigma-Aldrich

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

Synonyme(s) :

Urate oxidase

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About This Item

Numéro CAS:
9002-12-4
Numéro de classification (Commission des enzymes):
1.7.3.3 (BRENDA, IUBMB)
Numéro MDL:
MFCD00082126
Code UNSPSC :
12352204
ID de substance PubChem :
57653596
Nomenclature NACRES :
NA.54

Source biologique

Bacillus sp. (B. fastidiosus)

Forme

powder

Qualité

lyophilized

Activité spécifique

~9 U/mg

Conditions de stockage

dry at room temperature

Concentration

90—100%

Couleur

white to faint beige

Solubilité

5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)

Application(s)

detection

Température de stockage

−20°C

Chaîne SMILES 

N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

Clé InChI

VPXDHZMLJOJKOX-UHFFFAOYSA-N

Description générale

Research area: Cell signaling
Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Application

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Actions biochimiques/physiologiques

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Définition de l'unité

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Autres remarques

For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H319,H334,H360FD

Classification des risques

Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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U. Naegele et al.
Methods of Enzymatic Analysis, 7, 140-140 (1985)
M.H. Jensen et al.
Methods of Enzymatic Analysis, 7, 101-101 (1985)
Patrizia Zaramella et al.
Pediatrics, 131(1), e309-e312 (2012-12-05)
This case report describes a preterm newborn infant who was treated with a single dose of rasburicase for an increase in uric acid level. He died on the third day as a result of complications of hemolysis, which appeared to
Sara Cipriani et al.
Journal of neurochemistry, 123(1), 172-181 (2012-06-08)
Urate is the end product of purine metabolism and a major antioxidant circulating in humans. Recent data link higher levels of urate with a reduced risk of developing Parkinson's disease and with a slower rate of its progression. In this
Chia-Ter Chao et al.
Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 21(5), 498-500 (2012-06-13)
To report a case of huge hepatocellular carcinoma associated with tumor lysis syndrome and its management. A 51-year-old hepatitis B carrier visited our clinic with progressive weight loss over recent months. Abdominal ultrasonography and magnetic resonance imaging revealed a huge
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