SEQR

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

• Synonyme(s) : WGA kit, Whole transcriptome amplification
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.55

Propriétés

• Niveau de qualité: 200
• Technique(s): whole genome amplification: suitable
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

The SeqPlex^™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Application

SeqPlex^™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Caractéristiques et avantages

• Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
• Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
• No need to fragment DNA before sequencing.
• Amplifies ds-cDNA in 8 hours or less.
• Compatible with all next generation sequencing platforms except Pacific Bioscience.

Autres remarques

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Informations légales

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable