Accéder au contenu
Merck
Toutes les photos(2)

Principaux documents

Voir toute la documentation

WGA1

Sigma-Aldrich

GenomePlex® Whole Genome Amplification (WGA) Kit

Kit for whole genome amplification from a variety of DNA sources including FFPE tissue

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
41121800
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Technique(s)

whole genome amplification: suitable

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

GenomePlex® Whole Genome Amplification WGA kit is suitable for use with genomic DNA, whole blood, buffy coats, buccal swabs, and cultured cells. Amplification requires only a small amount of starting material (1 μL blood, single cheek swab, or 10 ng of gDNA), which after PCR produces a yield of 5 - 10 μg per reaction. The amplified DNA can be purified and stored or used for downstream analyses including ABI′s TaqMan® assays, microsatellite analysis, or sequencing. GenomePlex gives a complete representation of the entire genome with no detectable allele bias.

WGA Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

GenomePlex method allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The amplification yield is dependent on the purity and amount of starting material. This kit contains all the required reagents to perform library preparation and fragmentation.

Application

GenomePlex® Whole Genome Amplification (WGA) Kit has been used to amplify input and chromatin immunoprecipitation (ChIP) DNA. It has also been used in the fragmentation of bacterial artificial chromosome (BAC) clones from bacterial cultures for the preparation of repeat free (RF) probes.

Caractéristiques et avantages

  • Higher yield from minimal template: Amplification of nanogram amounts (10ng) of genomic DNA to microgram yields (5-10 μg) in less than about three hours
  • Nanograms of samples can be preserved at –20 °C for future use
  • Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, and formalin-fixed paraffin-embedded tissue (FFPE)
  • Compatible with a wide variety of downstream applications such as TaqMan® and BeadArray assays
  • Universal primers
  • Whole-genome representation with no detectable bias
  • Increased accuracy in amplification produces no amplicon in the negative control reactions

Autres remarques

The sequences of the universal primers provided in this kit are considered proprietary.

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologyFDS

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Adam Hittelman et al.
Diagnostic molecular pathology : the American journal of surgical pathology, part B, 16(4), 198-206 (2007-11-29)
Genome-based technologies such as genomic arrays and next generation sequencing are poised to make significant contributions to clinical oncology. However, translation of these technologies to the clinic will require that they produce high-quality reproducible data from small archived tumor specimens
Amy Breman et al.
Prenatal diagnosis, 32(4), 351-361 (2012-04-03)
To evaluate the results of prenatal chromosomal microarray analysis (CMA) on >1000 fetal samples referred for testing at our institution and to compare these data to published reports. High resolution CMA was offered to women undergoing amniocentesis or chorionic villus
Kaye N Ballantyne et al.
Forensic science international, 166(1), 35-41 (2006-05-12)
The analysis of LCN or highly degraded DNA samples presents a challenge for forensic science. Improving the quantity and/or quality of samples would greatly increase the profiling success rate from LCN and degraded samples. Whole genome amplification (WGA) is one
Nicola A Johnson et al.
Laboratory investigation; a journal of technical methods and pathology, 86(9), 968-978 (2006-06-06)
Array-based comparative genomic hybridisation (aCGH) has diverse applications in cancer gene discovery and translational research. Currently, aCGH is performed primarily using high molecular weight DNA samples and its application to formalin-fixed and paraffin-embedded (FFPE) tissues remains to be established. To
Binding patterns of BCL11A in the globin and GATA1 loci and characterization of the BCL11A fetal hemoglobin locus
Jawaid K, et al.
Blood Cells, Molecules and Diseases, 45(2), 140-146 (2010)
Afficher tous les articles scientifiques apparentés

Protocoles

Whole Gene Amplification Formalinfixed Paraffin embedded Tissues

Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases.

FFPE Tissue

Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.

Protocol for DNA extraction & WGA Amplification from Soil Samples

Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the GenomePlex® Whole Genome Amplification Protocol is followed

GenomePlex Whole Genome Amplification Kit Protocol (WGA1)

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

Afficher tout

Contenu apparenté

GenomePlex Whole Genome Amplification Kit Protocol (WGA1)

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique