R7397
ProtectRNA™ RNase Inhibitor 500× Concentrate
RNase inhibitor for in situ hybridization assays
Synonyme(s) :
Ribonuclease inhibitor ProtectRNA™
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41106300
Nomenclature NACRES :
NA.52
Produits recommandés
Forme
liquid
Température de stockage
2-8°C
Description générale
Not recommended in systems where other enzymatic activity is required.
ProtectRNA™ ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.
ProtectRNA™ ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.
Application
A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.
ProtectRNA™ RNase Inhibitor 500× Concentrate has been used in:
- RNA extraction
- tissue sectioning and staining for laser capture microscopy
- the isolation of blood vessels from hippocampi
ProtectRNA™ RNase Inhibitor 500× Concentrate is an RNase inhibitor.
Caractéristiques et avantages
- The 500× concentrate is economical
- Eliminates the need for special dishwashing and“after washing” cleaning and treatments.
Quantité
2mL treats 1,000ml of solution.
Autres remarques
This product is for R&D use only, not for drug, household, or other uses.
Informations légales
ProtectRNA is a trademark of Sigma-Aldrich Co. LLC
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Flam. Liq. 3 - Met. Corr. 1
Code de la classe de stockage
3 - Flammable liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
140.0 °F - closed cup
Point d'éclair (°C)
60 °C - closed cup
Listes réglementaires
Les listes réglementaires sont principalement fournies pour les produits chimiques. Seules des informations limitées peuvent être fournies ici pour les produits non chimiques. L'absence d'indication signifie qu'aucun des composants n'est répertorié. Il incombe à l'utilisateur de s'assurer de l'utilisation sûre et légale du produit.
EU REACH Annex XVII (Restriction List)
CAS No.
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Exposing the Three-Dimensional Biogeography and Metabolic States of Pathogens in Cystic Fibrosis Sputum via Hydrogel Embedding, Clearing, and rRNA Labeling.
DePas WH
mBio, 7(5), e00796-e00716 (2016)
Gene expression analysis of interferon kappa in laser capture microdissected cervical epithelium.
DeCarlo CA
Analytical Biochemistry, 381(1), 59-66 (2008)
Recovery of high-quality RNA from laser capture microdissected human and rodent pancreas.
Butler AE
Journal of Histotechnology, 39(2), 59-65 (2016)
Beatrice Quevedo et al.
BMC microbiology, 11, 14-14 (2011-01-21)
The purpose of this study was to design and evaluate fluorescent in situ hybridization (FISH) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli
David Greenwood et al.
Proteomics. Clinical applications, 14(3), e2000011-e2000011 (2020-03-31)
Periodontitis is linked to a localized dysbiotic microbial shift. This trending may often not be evident due to deep taxonomic changes of low abundance organisms and lack of consideration of variations in the treatment response. By using next generation sequencing
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique