GE17-1178-01
Resource™ S
Cytiva 17-1178-01, pack of 1 mL
Synonyme(s) :
Resource S Column
About This Item
Produits recommandés
ligand
sulfonate group
Description
Ion Exchanger Type (value)
Conditionnement
pack of 1 mL
Fabricant/nom de marque
Cytiva 17-1178-01
Paramètres
218 psi
Taille du lit
6.4 mm × 30 mm
Volume du lit
1 mL
Matrice
polystyrene/divinylbenzene
Taille moy. des particules
15 μm
Taille des particules
15-15 μm
cleaning
1-14
working range
2-13
Adéquation
suitable for bioprocess medium (BioProcess medium. Columns not suitable for use with ÄKTAprime plus system.)
suitable for bioprocess medium
Description générale
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Caractéristiques et avantages
- SOURCE™ 15Q and 15S media are well suited for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
This page shows how to perform a purification of His-tagged membrane proteins.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocoles
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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