G5080
Sephadex® G-50
Fine
Synonyme(s) :
Gel filtration resin, Sephadex G-50 Fine Medium
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About This Item
Produits recommandés
Technique(s)
affinity chromatography: suitable
Groupe de la matrice active
phase
Gonflement
1 g swells to 9-11 mL
Taille des billes
20-80 μm
Application(s)
life science and biopharma
Compatibilité
Cytiva
Description générale
Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine.
Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatography, and gel filtration chromatography. Sephadex is a beaded gel prepared by crosslinking dextran with epichlorohydrin.
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000
Application
Sephadex® G-50 has been used:
- to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension
- in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography
- in desalting the recombinant enzymes eluted in the fraction five and six
Sephadex® G-50 is suitable for use in:
- the separation of low and high molecular weight molecules
- affinity chromatography, protein chromatography, and gel filtration chromatography
Caractéristiques et avantages
- Desalts, removes contaminants, and transfers to a new buffer in one step.
- Suitable for DNA purification from small molecules using gel filtration.
- Features a small bead size, resulting in shorter diffusion distances.
- Considered a classic gel filtration resin.
- Desalting with Sephadex is considered superior to dialysis because it saves time, has a low dilution factor, and recovers high activity even with minute amounts of sample
Autres remarques
G5080-100G′s updated product number is GE17-0042-01
G5080-500G′s updated product number is GE17-0042-02
G5080-500G′s updated product number is GE17-0042-02
Informations légales
Sephadex is a registered trademark of Cytiva
Remplacé(e)(s) par
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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