GE17-1179-01
Resource™ Q
Cytiva 17-1179-01, pack of 6 mL
Synonyme(s) :
Resource Q Column
About This Item
Produits recommandés
ligand
quaternary ammonium
Description
Ion Exchanger Type (value)
Conditionnement
pack of 6 mL
Fabricant/nom de marque
Cytiva 17-1179-01
Paramètres
87 psi
Taille du lit
16 mm × 30 mm
Volume du lit
6 mL
Matrice
polystyrene/divinylbenzene
Taille moy. des particules
15 μm
Taille des particules
15-15 μm
cleaning
1-14
working range
2-12
Adéquation
suitable for bioprocess medium
Catégories apparentées
Description générale
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Application
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
Caractéristiques et avantages
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocoles
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.
This page discusses how to do a purification with SOURCE™ media from GE Healthcare
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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