DIGUTP-RO
Roche
Digoxigenin-11-UTP
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About This Item
Code UNSPSC :
41105500
Nomenclature NACRES :
NA.21
Produits recommandés
Essai
≥85% (HPLC)
Niveau de qualité
Forme
solution
Poids mol.
(Mr = 1106.7 (DIG-11-UTP-Li4))
Conditionnement
pkg of 25 μL (11209256910 [10 mM])
pkg of 57 μL (03359247910 [3.5 mM])
Fabricant/nom de marque
Roche
Température de stockage
−20°C
Description générale
Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.
Application
Digoxigenin-11-UTP has been used for labeling RNA probes in:
- in situ hybridization
- in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
- fluorescence in situ hybridization (FISH)
Actions biochimiques/physiologiques
Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.
Qualité
Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral
Code de la classe de stockage
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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