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11685619910

Roche

Fluorescein RNA Labeling Mix

solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization

Synonyme(s) :

RNA labeling

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About This Item

Code UNSPSC :
41105500

Forme

solution

Niveau de qualité

100

Utilisation

sufficient for 20 reactions

Conditionnement

pkg of 40 μL

Fabricant/nom de marque

Roche

Technique(s)

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

Température de stockage

−20°C

Catégories apparentées

Description générale

Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).

Spécificité

Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).

Application

Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
  • in situ hybridization to tissues
  • Southern blots
  • northern blots
  • plaque or colony lifts
  • RNase protection experiments

Qualité

The mix is function tested in combination with the DIG RNA Labeling Kit.

Principe

Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.

Notes préparatoires

10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

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