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11333089001

Roche

Anti-Digoxigenin

from sheep

Synonyme(s) :

anti-digoxigenin, digoxigenin

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About This Item

Code UNSPSC :
12352203

Source biologique

sheep

Niveau de qualité

100

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

lyophilized

Conditionnement

pkg of 200 μg

Fabricant/nom de marque

Roche

Isotype

IgG

Température de stockage

2-8°C

Description générale

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.

Spécificité

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Application

Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot

Actions biochimiques/physiologiques

In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.

Notes préparatoires

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.

Reconstitution

Add 1 ml PBS to a final concentration of 200 μg/ml.

Remarque sur l'analyse

No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+?ATPase by N?Hydroxysuccinimidyl Derivatives of Digoxigenin.
Antolovic R, et al.
European Journal of Biochemistry, 227(1?2), 61-67 (1995)
Alistair Nicol Boettiger et al.
Cell reports, 3(1), 8-15 (2013-01-29)
Transcription is commonly held to be a highly stochastic process, resulting in considerable heterogeneity of gene expression among the different cells in a population. Here, we employ quantitative in situ hybridization methods coupled with high-resolution imaging assays to measure the
Methods in Molecular Biology (2011)
Electron paramagnetic resonance of a single NV nanodiamond attached to an individual biomolecule.
Teeling-Smith R M, et al.
Biophysical Journal, 110(9), 2044-2052 (2016)
Jonathan Silver et al.
Biosensors & bioelectronics, 63, 117-123 (2014-07-30)
We describe a proof-of-principle, immune sandwich assay in which immune complexes link micron-size beads via DNA tethers to a sensor surface. The number of tethered beads, counted using low-magnification microscopy, provides a measure of the concentration of analyte. The prototype
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