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11207750910

Roche

Anti-Digoxigenin-Rhodamine, Fab fragments

from sheep

Synonyme(s) :

anti-digoxigenin, digoxigenin

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About This Item

Code UNSPSC :
12352203

Source biologique

sheep

Niveau de qualité

100

Conjugué

rhodamine conjugate

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

lyophilized (clear, red solution after reconstitution)

Conditionnement

pkg of 200 μg

Fabricant/nom de marque

Roche

Isotype

IgG

Température de stockage

2-8°C

Catégories apparentées

Description générale

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to rhodamine.

Spécificité

Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).

Application

Use Anti-Digoxigenin-Rhodamine, Fab fragments for the detection of digoxigenin-labeled compounds using:
  • Digoxigenin-labeled sugars in glycoconjugate research
  • Fluorescent in situ hybridization (FISH)
  • Immunohistocytochemistry
  • In situ hybridization
  • DNA fiber assay

Notes préparatoires

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
  • Fluorescent in situ hybridization (FISH): 1 to 20 μg/ml
  • Immunohistocytochemistry: 20 to 50 μg/ml
  • In situ hybridization: 20 to 50 μg/ml

Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.

Storage conditions (working solution): Always prepare fresh!

Reconstitution

Add 1 ml double-distilled water to a final concentration of 200 μg/ml.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317,H412

Classification des risques

Aquatic Chronic 3 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Spencer W Luebben et al.
Nucleic acids research, 41(22), 10283-10297 (2013-09-06)
HELQ is a superfamily 2 DNA helicase found in archaea and metazoans. It has been implicated in processing stalled replication forks and in repairing DNA double-strand breaks and inter-strand crosslinks. Though previous studies have suggested the possibility that HELQ is
Galina Pendinen et al.
Genome, 51(9), 714-720 (2008-09-06)
Thirty-six percent of the wild potato (Solanum L. section Petota Dumort.) species are polyploid, and about half of the polyploids are tetraploid species (2n = 4x = 48). Determination of the type of polyploidy and development of the genome concept
Olga G Silkova et al.
Plants (Basel, Switzerland), 10(10) (2021-10-24)
To date, few data have been accumulated on the contribution of meiotic restitution to the formation of Triticum aestivum hybrid karyotypes. In this study, based on FISH and C-banding, karyotype reorganization was observed in three groups of F5 wheat-rye hybrids
Shin-Ichiro Takebayashi et al.
Epigenetics & chromatin, 6(1), 42-42 (2013-12-18)
Cellular differentiation and reprogramming are accompanied by changes in replication timing and 3D organization of large-scale (400 to 800 Kb) chromosomal domains ('replication domains'), but few gene products have been identified whose disruption affects these properties. Here we show that
Xiaochun Wu et al.
The FEBS journal, 285(21), 3945-3958 (2018-09-07)
The krüppel-like factors (KLFs) are a family of transcription factor proteins that regulate a wide range of biological processes. In an RNAi-based screening experiment, we identified dendritic arbor reduction 1 (Dar1), which is a KLF member in Drosophila, that inhibited
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