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Key Documents

11093070910

Roche

Biotin-16-dUTP

Synonyme(s) :

Bio-16-dUTP tetralithium salt, Biotin-labeled Uridines, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′-deoxyuridine 5′-triphosphate

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About This Item

Formule empirique (notation de Hill):
C32H52N7O18P3S
Numéro CAS:
Poids moléculaire :
947.78
Code UNSPSC :
41116100

Forme

solution

Niveau de qualité

Conditionnement

pkg of 50 μL (50 nmol; 1mM)

Fabricant/nom de marque

Roche

Couleur

colorless

Solubilité

water: miscible

Température de stockage

−20°C

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Description générale

Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.Biotin-16-dUTP is used to produce biotinylated DNA probes that are commonly used to detect specific DNA and RNA sequences in different methods like Southern and Northern blotting, in situ hybridization, and sandwich assays.

Application

Biotin-16-dUTP has been used:
  • For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation. Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
  • as a substrate for terminal transferase, DNA polymerase I (holoenzyme and Klenow fragment), Taq DNA polymerase, and reverse transcriptase (e.g., Transcriptor). The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling
  • In fluorescence in situ hybridization (FISH)

Actions biochimiques/physiologiques

Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.

Qualité

Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).

Remarque sur l'analyse

Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3):
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.

Autres remarques

Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate- Biotin Luminescence Detection Kit.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Sudhanshu Agrawal et al.
American journal of physiology. Cell physiology, 311(3), C386-C391 (2016-07-15)
The water-soluble biotin (vitamin B7) is indispensable for normal human health. The vitamin acts as a cofactor for five carboxylases that are critical for fatty acid, glucose, and amino acid metabolism. Biotin deficiency is associated with various diseases, and mice
Zheng Li et al.
Nature, 583(7817), 625-630 (2020-07-17)
The recent discovery of N6-methyladenine (N6-mA) in mammalian genomes suggests that it may serve as an epigenetic regulatory mechanism1. However, the biological role of N6-mA and the molecular pathways that exert its function remain unclear. Here we show that N6-mA
Ling Hu et al.
Frontiers in cell and developmental biology, 9, 645368-645368 (2021-07-09)
Schizophrenia (SCZ) is a chronic and severe mental disease that affects around 1% of the population. The precise etiology of SCZ still remains largely unknown, and no conclusive mechanisms are firmly established. Recent advances in epidemiological and clinical investigation support
Di Hu et al.
Journal of neurotrauma, 37(21), 2244-2260 (2020-06-20)
Individuals with spinal cord injury (SCI) often develop debilitating neuropathic pain, which may be driven by neuronal damage and neuroinflammation. We have previously demonstrated that treatment using 670 nm (red) light irradiation alters microglia/macrophage responses and alleviates mechanical hypersensitivity at 7
Gadi Lalazar et al.
Cancer discovery, 11(10), 2544-2563 (2021-06-16)
To repurpose therapeutics for fibrolamellar carcinoma (FLC), we developed and validated patient-derived xenografts (PDX) from surgical resections. Most agents used clinically and inhibitors of oncogenes overexpressed in FLC showed little efficacy on PDX. A high-throughput functional drug screen found primary

Protocoles

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