IM29L

Anti-PAI-1 (Ab-1) Mouse mAb (2)

lyophilized, clone 2, Calbiochem^®

• Synonyme(s) : Anti-Plasminogen Activator Inhibitor-1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2, monoclonal
• Forme: lyophilized
• Ne contient pas: preservative
• Espèces réactives: human
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Isotype: IgG1
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SERPINE1(5054)

Description

Description générale

Purified mouse monoclonal antibody (see application references). Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.

Recognizes both active and latent forms of the ~50 kDa PAI-1 protein in conditioned media from dexamethasone-treated HT-1080 cells. Sold under license of U.S. Patent 5,422,245.

This Anti-PAI-1 (Ab-1) Mouse mAb (2) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of PAI-1 (Ab-1).

Immunogène

Human

partially purified PAI-1 from conditioned medium of dexamethasone-treated HT1080 human cells

Application

Immunoblotting (1 µg/ml)

Immunoprecipitation (see application references)

Immunocytochemistry (see application references

Neutralization Studies (see application references)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Remarque sur l'analyse

Positive Control
Conditioned media from dexamethasone-treated (1 µM) HT-1080 cells

Autres remarques

Antibody should be titrated for optimal results in individual systems.

Janicke, F., et al. 1994. Cancer Research.54, 2527.
Lawrence, D. A., et al. 1994. J. Biol. Chem.269, 15223.
Nagayama, M., et al. 1994. Cancer73, 1398.
Petersen, H., et al. 1994. Cancer Res.54, 120.
Keijer, J., et al. 1991. Blood78, 401.
Blasi, F. and Verde, P., 1990. in Seminars in Cancer Biol.Vol. 1, 153.
Declerck, P. J. and Collen, D., 1990. Thrombosis Res.Supplement X 3.
Nielsen, L. S., et al. 1986. Thrombosis and Haemostasis.55, 206.

Informations légales

Sold under license of U.S. Patent 5,422,245.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1