ABT1340

Anti-Myosin-10/MYH10/Myosin IIB Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Myosin-10, Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain Iib, MHC IIB
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, mouse
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_005955
• Numéro d'accès UniProt: P35580
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MYH10(4628)

Description

Description générale

Myosin-10 (UniProt P35580; also known as Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIb) is encoded by the MYH10 (also known as NMMHCB) gene (Gene ID 4628) in human. Nonmuscle myosin II plays an important role in cell migration and invasion. The myosin II holoenzyme consists of two myosin heavy chains (MHCs), two essential light chains, and two regulatory light chains. Three different nonmuscle MHC II proteins exist in mammals, IIA (MYH9), IIB (MYH10), and IIC (MYH14), with both unique and overlapping roles. One of the initial steps in cancer cell metastasis is thought to be the process of epithelial–mesenchymal transition (EMT). Myosin IIB expression and myosin IIA heavy chain phosphorylation are upregulated upon TGF-β–induced EMT in mammary epithelial cells, while inhibition of myosin IIB expression in post-EMT mesenchymal cells reduces transmigration and invasion.

Spécificité

Expected to react with all five spliced isoforms of human Myosin-10 reported by UniProt (P35580).

Immunogène

KLH-conjugated linear peptide corresponding to the C-terminal sequence of human myosin-10.

Application

Anti-Myosin-10/MYH10/Myosin IIB Antibody is an antibody against Myosin-10/MYH10/Myosin IIB for use in Western Blotting, Immunocytochemistry, Immunofluorescence.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Myosin-10/MYH10/Myosin IIB in 10 µg of MCF-10A cell lysate.
Western Blotting Analysis: A representative lot detected elevated MHC IIB expression in MDA-MB-468, MDA-MB-231, and MCF10A, while very low MHC IIB expression was detected in MCF7, T47D, BT-474, and SKBR3 (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Western Blotting Analysis: A representative lot detected MHC IIB levels in NMuMG murine mammary gland cells upon TGF-beta stimulation or shRNA-mediated hnRNP E1 knockdown. E1 overexpression obolished TGF-beta stimulated MHC IIB upregulation in NMuMG cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunocytochemistry Analysis: A representative lot detected MHC IIB immunoreactivity localized to the rear and perinuclear regions of migrating and transmigrating hnRNP E1-knockdown murine mammary gland E1-shRNA NMuMG cells by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% TritonX-100-permeablized cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunofluorescence Analysis: A representative lot detected MHC IIB immunoreactivity predominantly in the SAM-expressing myoepithelial layer, but not the cytokeratin 8-expressing luminal layer, in 4% paraformaldehyde-fixed, paraffin-embedded mouse mammary tissue sections by fluorescent immunohistochemstry (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).

Qualité

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myosin-10/MYH10/Myosin IIB in 10 µg of A549 cell lysate.

Description de la cible

~250 kDa observed. 229.0 kDa (isoform 1), 230.8 kDa (isoform 2), 231.4 kDa (isoform 3), 232.5 kDa (isoform 4), 230.0 kDa (isoform 5) calculated. Uncharacterized band(s) may appear in some lysates.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable