M7939

Anti-Myosin IIB, non-muscle antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-Myosin-10
• Numéro MDL: MFCD03455611
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~200 kDa
• Espèces réactives: rat, human, canine
• Technique(s): western blot (chemiluminescent): 1:200 using whole cell extracts of cultured dog MDCK kidney cells, cultured rat NRK cells, and cultured human Jurkat cells.
• Numéro d'accès UniProt: P35580
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: rat ... Myh10(79433)

Description

Description générale

Myosin IIB (nonmuscle) is a relatively abundant, widespread two headed myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled-coil region, and a nonhelical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. Myosin IIB is expressed in embryonic cardiac myocytes.

Spécificité

Anti-Myosin IIB, non-muscle antibody is specific for the heavy chain of human myosin IIB (nonmuscle). The antibody also reacts with myosin in dogs and rats.

Immunogène

Synthetic peptide corresponding to amino acid residues 1965-1976 of the heavy chain of human myosin IIB (nonmuscle) conjugated to KLH. This sequence is identical in rabbit and differs by one and two amino acids from the respective bovine and rat sequences.

Application

Anti-Myosin IIB, non-muscle antibody produced in rabbit has been used in:

• immunoblotting
• stimulated emission depletion (STED) nanoscopy
• immunofluorescence

Actions biochimiques/physiologiques

Myosin IIB plays a key role in the COS-7 cell cytokinesis and is need for the initiation of ring constriction.

Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin IIb regulates memory and synaptic plasticity functions. Nonmuscle myosin IIb has been implicated in fibroblast movement and erythroblast enucleation .

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable