GC Analysis of Pesticides in Fruits and Vegetables on a 14% Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb™
Materialien
Analytische Säule
Anwendung
SPE-Röhrchen oder -Platte
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
extract from 50 g homogenized fruit or vegetable sample
SPE tube/cartridge
ENVI-Carb, 500 mg/6 mL (57094)
sample addition
0.5 mL concentrated sample extract
elution
20 mL acetonitrile:toluene (3:1)
eluate post-treatment
Concentrate to 2 mL by rotary evaporation. Add 2 x 10 mL acetone concentrating to 2 mL after each acetone addition. (Add 50 μL I.S. (500 ng/μL cis-chlordane in acetone) then dilute to 2.5 mL with acetone.)
column
14% cyanopropylphenyl polysiloxane, 30m × 0.25 mm I.D., 0.15 μm (Supelco equivalent, Equity-1701, 28372-U)
oven
70 °C (hold 2 min) to 130 °C at 25 °C; 130 °C to 220 °C at 2 °C/min; 220 °C to 280 °C at 10 °C/min and hold 4.6 min
detector
MSD, 285 °C
carrier gas
helium
injection
2 μL, splitless
Beschreibung
Anwendung
Sample Pre-treatment:
Homogenize 50 g chopped fruit/vegetable sample in 100 mL acetonitrile. Add 10 g sodium chloride and homogenize 5 min. Transfer 13 mL of acetonitrile layer to clean centrifuge tube. Centrifuge at high speed for 5 min. Evaporate 10 mL aliquot of supernatant to 0.5 mL under Nitrogen at 35°C.
Rechtliche Hinweise
ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC