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Merck

ZMS1035

Sigma-Aldrich

Anti-MAG Antibody, clone 513 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(e):

Myelin-associated glycoprotein, Siglec-4a

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Rekombinant

expressed in HEK 293 cells

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

513, recombinant monoclonal
monoclonal

Produktlinie

ZooMAb® learn more

Form

lyophilized

Mol-Gew.

calculated mol wt 69.07 kDa
observed mol wt ~90 kDa

Speziesreaktivität

rat, mouse

Speziesreaktivität (Voraussage durch Homologie)

human, chicken, bovine, frog

Verpackung

antibody small pack of 25 μL

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Erweiterte Validierung

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Methode(n)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotyp

IgG1

Grünere Alternativprodukt-Kategorie

Versandbedingung

ambient

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... MAG(4099)

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spezifität

Clone 513 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Myelin-associated glycoprotein.

Immunogen

L2 epitope-carrying glycoproteins from 1- to 2- day-old chicken brains.

Anwendung

Anti-MAG, clone 513 ZooMAb, Cat. No. ZMS1035, s a recombinant Mouse monoclonal antibody that targets Myelin Associated Glycoprotein and is tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MAG in rat brain tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MAG in rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MAG in Oligodendrocyte precursor cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Zielbeschreibung

Myelin-associated glycoprotein (Siglec-4a, MAG) is encoded by the MAG (also known as GMA) gene (Gene ID: 4099) in chicken. MAG is a single-pass type I membrane protein that is expressed in myelin-forming cells when oligodendrites initiate contact with axons. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-516), a transmembrane domain (aa 517-536), and a cytoplasmic domain (aa 518-626). MAG is a member of the immunoglobulin superfamily that is found in both peripheral and CNS myelin, where it plays a role in the formation and maintenance of myelin sheath. It stabilizes contacts between myelinating axons and glial processes. It also participates in the formation of morphologically intact myelin sheath that results in periaxonal cytoplasmic collar of Schwann cells and compact myelin structure of oligodentrocytes. MAG is also considered to be important in maintenance of myelin with aging. MAG protects motoneurons against apoptosis and injury that is mediated via its interaction with neuronal reticulon receptor 4R (RTN4R) and Reticulon-4 receptor-like 2 protein (RTN4RL2). Three isoforms of MAG protein have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Poltorak, M., et al. (1987). J Cell Biol. 105(4); 1893-1899; Quarles, RH. (2007). J. Neurochem. 100(6); 1431-1448).

Physikalische Form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstituierung

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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