T6424

Trypsin aus meschlichem Pankreas

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

• Synonym(e): Serine Protease 1
• CAS-Nummer: 9002-07-7
• EC-Nummer: 3.4.21.4 (BRENDA, IUBMB)
• EG-Nummer: 232-650-8
• MDL-Nummer: MFCD01323069
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: human pancreas
• Qualitätsniveau: 200
• Form: salt-free, lyophilized powder
• Verpackung: vial of ≥1,000 BAEE units
• UniProt-Hinterlegungsnummer: P07477; P07478; P35030
• Lagertemp.: −20°C
• Angaben zum Gen: human ... PRSS1(5644) , PRSS2(5645) , PRSS3(5646)

Beschreibung

Allgemeine Beschreibung

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.

Anwendung

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.

Einheitendefinition

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Menge

Minimum 1,000 BAEE units/vial

Hinweis zur Analyse

Proteingehalt mittels E1%/280.

Sonstige Hinweise

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H315,H319,H334,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves