SRP0142

PRMT3 human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

• Synonym(e): Arginine methyltransferase 3, HMT1 hnRNP methyltransferase-like 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like 3
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥60% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 86 kDa
• Verpackung: pkg of 50 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_005788
• UniProt-Hinterlegungsnummer: O60678
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PRMT3(10196)

Beschreibung

Allgemeine Beschreibung

Human PRMT3, GenBank Accession No. NM_005788, amino acids 2-end, with N-terminal GST tag, MW = 86 kDa, expressed in an E. coli expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.