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Merck

S2454

Millipore

Seppro Ammonium Bicarbonate Buffer

Synonym(e):

ABC buffer, Ammonium hydrogen carbonate, Monoammonium carbonate

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.32

Form

solution

Qualitätsniveau

Konzentration

2 M

Methode(n)

protein expression: suitable

Farbe

clear colorless

Anwendung(en)

life science and biopharma

Lagertemp.

room temp

Allgemeine Beschreibung

Seppro® Ammonium Bicarbonate Buffer is an effective buffer for protein digestion and deglycosylation reaction. It may be used to coat 2D and 3D electrophoretic gel pieces during digestion of proteins and proteomes. The ammonium bicarbonate buffer also provides moisture during enzymatic cleavage. Ammonium bicarbonate buffer regulates the microenvironmental pH of immobilized cephalosporin C acylase (CCA) for the catalysis of cephalosporin C (CPC) in a batch stirred tank reactor (STR).

Anwendung

Ammonium bicarbonate buffer has been used as a component of cell lysis/protein extraction buffer for proteomic sample preparation.

Rechtliche Hinweise

6538 is a trademark of American Type Culture Collection
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Emily S Boja et al.
The Journal of biological chemistry, 278(36), 34189-34202 (2003-06-12)
The zona pellucida is an extracellular matrix consisting of three glycoproteins that surrounds mammalian eggs and mediates fertilization. The primary structures of mouse ZP1, ZP2, and ZP3 have been deduced from cDNA. Each has a predicted signal peptide and a
Andrej Shevchenko et al.
Nature protocols, 1(6), 2856-2860 (2007-04-05)
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel
Vimal K Balasubramanian et al.
Current protocols, 1(5), e153-e153 (2021-05-28)
Plant organs and tissues contain multiple cell types, which are well organized in 3-dimensional structure to efficiently perform physiological functions such as homeostasis and response to environmental perturbation and pathogen infection. It is critically important to perform molecular measurements at

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