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P1411

Sigma-Aldrich

Polymyxin-B-Agarose

aqueous glycerol suspension

Synonym(e):

PMB

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About This Item

MDL-Nummer:
MFCD00165491
UNSPSC-Code:
41106500
NACRES:
NA.56

Biologische Quelle

synthetic (organic)

Qualitätsniveau

200

Form

aqueous glycerol suspension

Kennzeichnungsgrad

~1 mg per mL

Methode(n)

affinity chromatography: suitable

Matrix

Cross-linked 4% beaded agarose

Matrixaktivierung

cyanogen bromide

Matrixanbindung

amino

Matrix-Spacer

1 atom

Kapazität

200-500 μg/mL binding capacity (lipopolysaccharide)(from Escherichia coli serotype 0128:B12)

Eignung

suitable for chromatography

Lagertemp.

2-8°C

Anwendung

Nützlich für Entfernung von Endotoxinen aus Lösungen
Polymyxin B-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polymyxin B-agarose has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes.

Physikalische Form

Suspension in 50% Glycerin, enthält 0.02% Natriumazid

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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J Gil et al.
Cancer research, 50(22), 7301-7306 (1990-11-15)
We have shown previously that IgM from Ehrlich tumor (ET)-immunized mice, recognizing ET cell surface carbohydrates, protects control mice to a subsequent tumor challenge. The factors involved in such IgM-mediated protection were unknown, since it was independent of complement activation.
A C Issekutz
Journal of immunological methods, 61(3), 275-281 (1983-07-29)
Endotoxins liberated by gram-negative bacteria are frequent contaminants of aqueous and physiological solutions. Because of their potent biological effects in vivo and in vitro, it is often necessary to eliminate even minute quantities of endotoxin from such solutions. A process
P J Jagielo et al.
The American journal of physiology, 270(6 Pt 1), L1052-L1059 (1996-06-01)
To investigate the role of endotoxin in grain dust-induced airway inflammation, we reduced the endotoxin activity from extracts of corn dust (CDE), using three distinct methods, and determined the effect of endotoxin activity on the in vitro and in vivo
K W Talmadge et al.
Journal of chromatography, 476, 175-185 (1989-08-04)
A new affinity column packing for removal of endotoxins has been prepared by coupling USP drug-quality polymyxin B to Affi-Prep, a novel synthetic macroporous polymer. Affi-Prep Polymyxin binds endotoxins from a number of different strains of gram-negative bacteria. Endotoxin binding
Martín López et al.
Journal of immunology (Baltimore, Md. : 1950), 170(5), 2409-2416 (2003-02-21)
Macrophages infected with Mycobacterium tuberculosis undergo increased rates of apoptosis. Important objectives are to define the microbial factors that cause apoptosis, the mechanisms involved and the impact on infection. The 19-kDa M. tuberculosis glycolipoprotein (p19) is both cell wall-associated and
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