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Merck

RAB0426

Sigma-Aldrich

Human P-Selectin ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(e):

Cytokine detection kit

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About This Item

UNSPSC-Code:
41116158
NACRES:
NA.32

Speziesreaktivität

human

Verpackung

kit of 96 wells (12 strips x 8 wells)

Methode(n)

ELISA: suitable
capture ELISA: suitable

Aufnahme

sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 0.041-30 ng/mL

Nachweisverfahren

colorimetric

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... SELP(6403)

Allgemeine Beschreibung

The Human P-Selectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human P-Selectin in serum, plasma, cell culture supernatants and urine.P-selectin, also known as cluster of differentiation 62 (CD62) or granule membrane protein 140 (GMP-140), is a Ca2+-dependent lectin. It is expressed within alpha-granules of platelets and the Weibel-Palade bodies of endothelial cells.

Immunogen

Recombinant Human P-Selectin

Anwendung

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Human P-Selectin ELISA (Enzyme-Linked Immunosorbent Assay) kit has been used in ELISA to quantify P-selectin levels in platelet lysates.
For research use only. Not for use in diagnostic procedures.

Biochem./physiol. Wirkung

P-selectin serves as an adhesion molecule and plays an important role in leukocyte rolling and attachment. In addition, it also acts a receptor for myeloid cells by associating with sialylated, fucosylated lactosaminoglycans. Overexpression of P-selectin has been observed in apparently healthy women at risk for future vascular events.

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDB

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDB

  • RABSTOP3ELISA Stop Solution (Item I)SDB

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDB

  • RABWASH420X Wash Buffer (Item B)SDB

Piktogramme

Corrosion

Signalwort

Warning

H-Sätze

P-Sätze

Gefahreneinstufungen

Met. Corr. 1

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Effects of selenium in the MAPK signaling cascade.
Rashtchizadeh N, et al.
Journal of cardiovascular and thoracic research, 7(3), 107-107 (2015)
Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells.
Moore K L, et al.
The Journal of Cell Biology, 118(2), 445-456 (1992)
Soluble P-selectin and the risk of future cardiovascular events.
Ridker P M, et al.
Circulation, 103(4), 491-495 (2001)
Nadereh Rashtchizadeh et al.
Journal of cardiovascular and thoracic research, 7(3), 107-112 (2015-10-03)
This study aimed to discover by which mechanism selenium (Se) suppresses stimulated platelets stimulation in oxidative stress underlying diseases. Human platelets pretreated with Se and stimulated by Cu(2+)-oxidized low density of lipoprotein (OxLDL) or thrombin before assessment of P-selectin and

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