P4300

Pektinase aus Rhizopus sp.

suitable for plant cell culture, BioReagent, crude powder

• Synonym(e): Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
• CAS-Nummer: 9032-75-1
• EC-Nummer: 3.2.1.15 (BRENDA, IUBMB)
• EG-Nummer: 232-885-6
• MDL-Nummer: MFCD00131809
• UNSPSC-Code: 12352200

Eigenschaften

• Produktlinie: BioReagent
• Form: crude powder
• Spezifische Aktivität: 400-800 units/g solid
• Methode(n): cell culture | plant: suitable
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Pectinase P4300 is a natural pectinase (polygalacturonase) derived from fungus Rhizops sp. that randomly hydrolyzes the (1→4)-α-D-galactosiduronic linkages in pectate and other galacturonans. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes. Pectinase P4300 is used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Anwendung

Zur Herstellung von Pflanzenprotoplast für den Zellwandabbau vor der organellen Isolation.

Einheitendefinition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)