P0609

Pepsin−Agarose from porcine gastric mucosa

lyophilized powder, ≥30 units/mg dry solid

• Synonym(e): Pepsin A
• EC-Nummer: 3.4.23.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00081840
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Porcine gastric mucosa
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥30 units/mg dry solid
• Mol-Gew.: 35 kDa
• Matrix: 4% cross-linked beaded agarose
• UniProt-Hinterlegungsnummer: P00791
• Lagertemp.: −20°C
• Angaben zum Gen: pig ... LOC396892(396892)

Beschreibung

Allgemeine Beschreibung

Pepsin is an enzyme found in gastric secretion.

Anwendung

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.

Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.

Used to produce F(ab′)₂ fragments of antibodies.¹

Biochem./physiol. Wirkung

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

Einheitendefinition

One unit will produce a ΔA₂₈₀ of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

Menge

One gram solid swells to 5-10ml packed gel.

Physikalische Form

Lyophilisiertes Pulver stabilisiert mit Lactose

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves