GE17-5072-01
Q Sepharose™ Xl
Cytiva 17-5072-01, pack of 300 mL
About This Item
Empfohlene Produkte
ligand
quaternary amine
Beschreibung
Ion Exchanger Type (value)
Verpackung
pack of 300 mL
Hersteller/Markenname
Cytiva 17-5072-01
Matrix
6% cross-linked agarose with dextran surface extenders
Partikelgröße
45-165 μm
Durchschnittlicher Durchmesser
90 μm
cleaning in place
2-14
working range
2-12
Eignung
suitable for bioprocess medium
Verwandte Kategorien
Allgemeine Beschreibung
Q Sepharose™ XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose™ Fast Flow media. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.
With its high loading capacities and excellent physical and chemical stability, Q Sepharose™ XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.
As member of the BioProcess media range, Q Sepharose™ XL meets industrial demands with security of supply and comprehensive technical and regulatory support.
Leistungsmerkmale und Vorteile
- High loading capacities give more productive capture from clarified feedstocks.
- Strong anion exchanger designed for capture steps
- High chemical stability for effective CIP/sanitization
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
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Artikel
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protokolle
This page covers how and when to use Sepharose XL media for purification of proteins.
This page discusses how to do a purification with SOURCE™ media from GE Healthcare
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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